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c4 mouse anti human lamb2 antibody  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank c4 mouse anti human lamb2 antibody
    Staining for the podocyte extracellular matrix proteins laminin-β2 <t>(LAMB2),</t> agrin, and collagen type IV-α2 (COL4A4) are increased in parietal epithelial cells (PECs) in experimental focal segmental glomerulosclerosis (FSGS). A−C: mice at baseline. <t>LAMB2</t> (A), agrin (B), and COL4A4 (C) immunofluorescent staining (green) was limited to the glomerular basement membrane (yellow arrowheads). Nuclei were stained with DAPI (blue). D−F: experimental FSGS in mice. De novo immunofluorescent staining for LAMB2 (D), agrin (E), and COL4A4 (F) was detected in PECs at day 28 (D28) FSGS (white arrows). G−I: quantification. The percentage of glomerular cross-sections with de novo staining for LAMB2 (G), agrin (H), and COL4A4 (I) increased at day 28 FSGS compared with baseline. The number of mice quantitated is shown below each graph. J−L: graphs of quantification of fluorescence intensity. Fluorescence intensity was increased for LAMB2 (J), agrin (K), and COL4A4 (L) in abnormal glomeruli in FSGS. Original magnification: ×400. Scale bars = 20 μm.
    C4 Mouse Anti Human Lamb2 Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 126 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c4 mouse anti human lamb2 antibody/product/Developmental Studies Hybridoma Bank
    Average 99 stars, based on 126 article reviews
    c4 mouse anti human lamb2 antibody - by Bioz Stars, 2026-05
    99/100 stars

    Images

    1) Product Images from "Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy"

    Article Title: Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy

    Journal: American Journal of Physiology - Renal Physiology

    doi: 10.1152/ajprenal.00266.2019

    Staining for the podocyte extracellular matrix proteins laminin-β2 (LAMB2), agrin, and collagen type IV-α2 (COL4A4) are increased in parietal epithelial cells (PECs) in experimental focal segmental glomerulosclerosis (FSGS). A−C: mice at baseline. LAMB2 (A), agrin (B), and COL4A4 (C) immunofluorescent staining (green) was limited to the glomerular basement membrane (yellow arrowheads). Nuclei were stained with DAPI (blue). D−F: experimental FSGS in mice. De novo immunofluorescent staining for LAMB2 (D), agrin (E), and COL4A4 (F) was detected in PECs at day 28 (D28) FSGS (white arrows). G−I: quantification. The percentage of glomerular cross-sections with de novo staining for LAMB2 (G), agrin (H), and COL4A4 (I) increased at day 28 FSGS compared with baseline. The number of mice quantitated is shown below each graph. J−L: graphs of quantification of fluorescence intensity. Fluorescence intensity was increased for LAMB2 (J), agrin (K), and COL4A4 (L) in abnormal glomeruli in FSGS. Original magnification: ×400. Scale bars = 20 μm.
    Figure Legend Snippet: Staining for the podocyte extracellular matrix proteins laminin-β2 (LAMB2), agrin, and collagen type IV-α2 (COL4A4) are increased in parietal epithelial cells (PECs) in experimental focal segmental glomerulosclerosis (FSGS). A−C: mice at baseline. LAMB2 (A), agrin (B), and COL4A4 (C) immunofluorescent staining (green) was limited to the glomerular basement membrane (yellow arrowheads). Nuclei were stained with DAPI (blue). D−F: experimental FSGS in mice. De novo immunofluorescent staining for LAMB2 (D), agrin (E), and COL4A4 (F) was detected in PECs at day 28 (D28) FSGS (white arrows). G−I: quantification. The percentage of glomerular cross-sections with de novo staining for LAMB2 (G), agrin (H), and COL4A4 (I) increased at day 28 FSGS compared with baseline. The number of mice quantitated is shown below each graph. J−L: graphs of quantification of fluorescence intensity. Fluorescence intensity was increased for LAMB2 (J), agrin (K), and COL4A4 (L) in abnormal glomeruli in FSGS. Original magnification: ×400. Scale bars = 20 μm.

    Techniques Used: Staining, Membrane, Fluorescence

    Double staining confirmed laminin-β2 (LAMB2) expression in parietal epithelial cells (PECs) in mice with experimental focal segmental glomerulosclerosis (FSGS). A and B: double staining for nephrin (red) and LAMB2 (green). A and A3: a normal mouse glomerulus. The merged image shows staining for LAMB2 (green) in the glomerular basement membrane distribution, with nephrin staining (red) alongside. A1 and A2: staining for nephrin (A1) and LAMB2 (A2) showing their absence along Bowman’s capsule. B and B3: FSGS. The merged image shows de novo LAMB2 but not nephrin staining along Bowman’s capsule. B1 and B2: nephrin (B1) and LAMB2 (B2) staining. Higher magnification of the inset in B showed no staining for nephrin (B4) along Bowman’s capsule in areas staining for LAMB2 (white arrows) (B5). Their merged image is shown in B6. C and D: double staining for β-Gal (red) and LAMB2 (green). C and C3: a normal glomerulus. The merged image shows that there was no overlap of LAMB2 staining in the glomerular basement membrane distribution and β-Gal, which permanently labels PECs. C1 and C2: β-Gal and LAMB2. D and D3: FSGS. The merged image shows de novo staining for LAMB2 in a Bowman’s capsule distribution (D1 and D2). Higher power magnification of the inset D shows that β-Gal (yellow arrows) (D4) is lined by LAMB2 (white arrows) (D5), as easily seen in the merged image (D6). Original magnification: ×400. Scale bars = 20 μm.
    Figure Legend Snippet: Double staining confirmed laminin-β2 (LAMB2) expression in parietal epithelial cells (PECs) in mice with experimental focal segmental glomerulosclerosis (FSGS). A and B: double staining for nephrin (red) and LAMB2 (green). A and A3: a normal mouse glomerulus. The merged image shows staining for LAMB2 (green) in the glomerular basement membrane distribution, with nephrin staining (red) alongside. A1 and A2: staining for nephrin (A1) and LAMB2 (A2) showing their absence along Bowman’s capsule. B and B3: FSGS. The merged image shows de novo LAMB2 but not nephrin staining along Bowman’s capsule. B1 and B2: nephrin (B1) and LAMB2 (B2) staining. Higher magnification of the inset in B showed no staining for nephrin (B4) along Bowman’s capsule in areas staining for LAMB2 (white arrows) (B5). Their merged image is shown in B6. C and D: double staining for β-Gal (red) and LAMB2 (green). C and C3: a normal glomerulus. The merged image shows that there was no overlap of LAMB2 staining in the glomerular basement membrane distribution and β-Gal, which permanently labels PECs. C1 and C2: β-Gal and LAMB2. D and D3: FSGS. The merged image shows de novo staining for LAMB2 in a Bowman’s capsule distribution (D1 and D2). Higher power magnification of the inset D shows that β-Gal (yellow arrows) (D4) is lined by LAMB2 (white arrows) (D5), as easily seen in the merged image (D6). Original magnification: ×400. Scale bars = 20 μm.

    Techniques Used: Double Staining, Expressing, Staining, Membrane

    The podocyte-specific extracellular matrix proteins laminin-β2 (LAMB2), agrin, and collagen type IV-α4 (COL4A4) increased in parietal epithelial cells (PECs) in human focal segmental glomerulosclerosis (FSGS). A–C: LAMB2 (green) and DAPI (blue). A: LAMB2 localized to the glomerular basement membrane (GBM) in the normal human kidney. B and C: two representative glomeruli from different patients with FSGS showing LAMB2 staining along Bowman’s capsule. B′ and C′: higher magnification of the insets showing LAMB2 staining (white arrows) along Bowman’s capsule. D–F: agrin (green) and DAPI. D: agrin localized to the GBM in a normal human glomerulus. E and F: two representative glomeruli from different patients with FSGS showing agrin staining along Bowman’s capsule. E′ and F′: higher magnification of the insets showing LAMB2 staining (white arrows) along Bowman’s capsule. G–I: COL4A4 (green) and DAPI. G: COL4A4 localized to the GBM in a normal human glomerulus. H and I: two representative glomeruli from different patients with FSGS showing COL4A4 staining along Bowman’s capsule. H′ and I′: higher magnification of the insets showing COL4A4 staining (white arrows) along Bowman’s capsule. These results show that podocyte-specific extracellular matrix proteins are expressed de novo by PECs in human FSGS. Original magnification: ×200. Scale bars = 100 μm.
    Figure Legend Snippet: The podocyte-specific extracellular matrix proteins laminin-β2 (LAMB2), agrin, and collagen type IV-α4 (COL4A4) increased in parietal epithelial cells (PECs) in human focal segmental glomerulosclerosis (FSGS). A–C: LAMB2 (green) and DAPI (blue). A: LAMB2 localized to the glomerular basement membrane (GBM) in the normal human kidney. B and C: two representative glomeruli from different patients with FSGS showing LAMB2 staining along Bowman’s capsule. B′ and C′: higher magnification of the insets showing LAMB2 staining (white arrows) along Bowman’s capsule. D–F: agrin (green) and DAPI. D: agrin localized to the GBM in a normal human glomerulus. E and F: two representative glomeruli from different patients with FSGS showing agrin staining along Bowman’s capsule. E′ and F′: higher magnification of the insets showing LAMB2 staining (white arrows) along Bowman’s capsule. G–I: COL4A4 (green) and DAPI. G: COL4A4 localized to the GBM in a normal human glomerulus. H and I: two representative glomeruli from different patients with FSGS showing COL4A4 staining along Bowman’s capsule. H′ and I′: higher magnification of the insets showing COL4A4 staining (white arrows) along Bowman’s capsule. These results show that podocyte-specific extracellular matrix proteins are expressed de novo by PECs in human FSGS. Original magnification: ×200. Scale bars = 100 μm.

    Techniques Used: Membrane, Staining

    Differential immunofluorescent immunostaining in parietal epithelial cells (PECs) for laminin-β2 (LAMB2), agrin, and collagen type IV-α4 (COL4A4) in an experimental diabetic nephropathy model. A−C: nondiabetic wild-type (WT) mice. Immunofluorescent staining for LAMB2 (A), agrin (B), and COL4A4 (C) staining (green) showed a podocyte distribution. Nuclei were stained with DAPI (blue). D−F: diabetic ob/ob mice. De novo staining was detected in PECs (white arrows) for LAMB2 (D), agrin (E), and COL4A4 (F) and in the mesangium (yellow arrows). G−I: quantification. The percentage of glomerular cross sections with de novo expression along Bowman’s capsule (BC) increased in diabetic mice for LAMB2 (G) and agrin (H) but not COL4A4 (I). Original magnification: ×400. Scale bars = 20 μm.
    Figure Legend Snippet: Differential immunofluorescent immunostaining in parietal epithelial cells (PECs) for laminin-β2 (LAMB2), agrin, and collagen type IV-α4 (COL4A4) in an experimental diabetic nephropathy model. A−C: nondiabetic wild-type (WT) mice. Immunofluorescent staining for LAMB2 (A), agrin (B), and COL4A4 (C) staining (green) showed a podocyte distribution. Nuclei were stained with DAPI (blue). D−F: diabetic ob/ob mice. De novo staining was detected in PECs (white arrows) for LAMB2 (D), agrin (E), and COL4A4 (F) and in the mesangium (yellow arrows). G−I: quantification. The percentage of glomerular cross sections with de novo expression along Bowman’s capsule (BC) increased in diabetic mice for LAMB2 (G) and agrin (H) but not COL4A4 (I). Original magnification: ×400. Scale bars = 20 μm.

    Techniques Used: Immunostaining, Staining, Expressing

    Laminin-β2 (LAMB2), agrin, and collagen type IV-α4 (COL4A4) staining in human diabetic nephropathy. A–C: LAMB2 staining. A: LAMB2 staining was restricted to the glomerular basement membrane (GBM) in the normal glomerulus. B and C: LAMB2 was detected along Bowman’s capsule in glomeruli from two different patients with diabetic nephropathy. B′ and C′: higher magnification of the insets showing de novo staining for LAMB2 along Bowman’s capsule (white arrows) and in the mesangium (yellow arrow). D–F: agrin staining. D: agrin staining localized to the GBM in the normal glomerulus. E and F: agrin was also detected along Bowman’s capsule in glomeruli from two different patients. E′ and F′: higher magnification of the insets showing staining for agrin along Bowman’s capsule (white arrows) and in the mesangium (yellow arrow). G–I: COL4A4 staining. G: COL4A4 staining localized to the GBM in the normal glomerulus. H and I: COL4A4 was also detected along Bowman’s capsule in glomeruli from two different patients with diabetic nephropathy. H′ and I′: higher magnification showing COL4A4 staining along Bowman’s capsule (white arrows) and in the mesangium (yellow arrow). Original magnification: ×200. Scale bars = 100 μm.
    Figure Legend Snippet: Laminin-β2 (LAMB2), agrin, and collagen type IV-α4 (COL4A4) staining in human diabetic nephropathy. A–C: LAMB2 staining. A: LAMB2 staining was restricted to the glomerular basement membrane (GBM) in the normal glomerulus. B and C: LAMB2 was detected along Bowman’s capsule in glomeruli from two different patients with diabetic nephropathy. B′ and C′: higher magnification of the insets showing de novo staining for LAMB2 along Bowman’s capsule (white arrows) and in the mesangium (yellow arrow). D–F: agrin staining. D: agrin staining localized to the GBM in the normal glomerulus. E and F: agrin was also detected along Bowman’s capsule in glomeruli from two different patients. E′ and F′: higher magnification of the insets showing staining for agrin along Bowman’s capsule (white arrows) and in the mesangium (yellow arrow). G–I: COL4A4 staining. G: COL4A4 staining localized to the GBM in the normal glomerulus. H and I: COL4A4 was also detected along Bowman’s capsule in glomeruli from two different patients with diabetic nephropathy. H′ and I′: higher magnification showing COL4A4 staining along Bowman’s capsule (white arrows) and in the mesangium (yellow arrow). Original magnification: ×200. Scale bars = 100 μm.

    Techniques Used: Staining, Membrane

    Podocyte-specific proteins colocalize with the activated parietal epithelial cell (PEC) marker CD44 in mice with experimental focal segmental glomerulosclerosis (FSGS). Double staining was performed for CD44 (red) and podocyte-specific extracellular matrix proteins (green). A−C: normal mice. The merged images show staining for laminin-β2 (LAMB2; A), agrin (B), and collagen type IV-α4 (COL4A4; C) but not CD44 in normal glomeruli. A1−C3: individual stains for LAMB2 (A1−A3), agrin (B1−B3), and COL4A4 (C1–C3). D−F: FSGS mice. The merged images show colocalization (yellow) of CD44 in PECs with LAMB2 (D and D3) and agrin (E and E3) but not COL4A4 (F and F3). D1, D2, E1, E2, F1, and F2: corresponding single stainings for LAMB2 (D1 and D2), agrin (E1 and E2), and COL4A4 (F1 and F2). Single and merged higher magnifications of the insets are shown in D4–F6). These results are consistent with activated PECs expressing the podocyte-specific extracellular matrix proteins LAMB2 and agrin in FSGS. Original magnification: ×400. Scale bars = 20 μm.
    Figure Legend Snippet: Podocyte-specific proteins colocalize with the activated parietal epithelial cell (PEC) marker CD44 in mice with experimental focal segmental glomerulosclerosis (FSGS). Double staining was performed for CD44 (red) and podocyte-specific extracellular matrix proteins (green). A−C: normal mice. The merged images show staining for laminin-β2 (LAMB2; A), agrin (B), and collagen type IV-α4 (COL4A4; C) but not CD44 in normal glomeruli. A1−C3: individual stains for LAMB2 (A1−A3), agrin (B1−B3), and COL4A4 (C1–C3). D−F: FSGS mice. The merged images show colocalization (yellow) of CD44 in PECs with LAMB2 (D and D3) and agrin (E and E3) but not COL4A4 (F and F3). D1, D2, E1, E2, F1, and F2: corresponding single stainings for LAMB2 (D1 and D2), agrin (E1 and E2), and COL4A4 (F1 and F2). Single and merged higher magnifications of the insets are shown in D4–F6). These results are consistent with activated PECs expressing the podocyte-specific extracellular matrix proteins LAMB2 and agrin in FSGS. Original magnification: ×400. Scale bars = 20 μm.

    Techniques Used: Marker, Double Staining, Staining, Expressing



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    c4 mouse anti human lamb2 antibody  (Developmental Studies Hybridoma Bank)
    99
    Developmental Studies Hybridoma Bank c4 mouse anti human lamb2 antibody
    Staining for the podocyte extracellular matrix proteins laminin-β2 <t>(LAMB2),</t> agrin, and collagen type IV-α2 (COL4A4) are increased in parietal epithelial cells (PECs) in experimental focal segmental glomerulosclerosis (FSGS). A−C: mice at baseline. <t>LAMB2</t> (A), agrin (B), and COL4A4 (C) immunofluorescent staining (green) was limited to the glomerular basement membrane (yellow arrowheads). Nuclei were stained with DAPI (blue). D−F: experimental FSGS in mice. De novo immunofluorescent staining for LAMB2 (D), agrin (E), and COL4A4 (F) was detected in PECs at day 28 (D28) FSGS (white arrows). G−I: quantification. The percentage of glomerular cross-sections with de novo staining for LAMB2 (G), agrin (H), and COL4A4 (I) increased at day 28 FSGS compared with baseline. The number of mice quantitated is shown below each graph. J−L: graphs of quantification of fluorescence intensity. Fluorescence intensity was increased for LAMB2 (J), agrin (K), and COL4A4 (L) in abnormal glomeruli in FSGS. Original magnification: ×400. Scale bars = 20 μm.
    C4 Mouse Anti Human Lamb2 Antibody, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/c4 mouse anti human lamb2 antibody/product/Developmental Studies Hybridoma Bank
    Average 99 stars, based on 1 article reviews
    c4 mouse anti human lamb2 antibody - by Bioz Stars, 2026-05
    99/100 stars
    		  Buy from Supplier

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    Staining for the podocyte extracellular matrix proteins laminin-β2 (LAMB2), agrin, and collagen type IV-α2 (COL4A4) are increased in parietal epithelial cells (PECs) in experimental focal segmental glomerulosclerosis (FSGS). A−C: mice at baseline. LAMB2 (A), agrin (B), and COL4A4 (C) immunofluorescent staining (green) was limited to the glomerular basement membrane (yellow arrowheads). Nuclei were stained with DAPI (blue). D−F: experimental FSGS in mice. De novo immunofluorescent staining for LAMB2 (D), agrin (E), and COL4A4 (F) was detected in PECs at day 28 (D28) FSGS (white arrows). G−I: quantification. The percentage of glomerular cross-sections with de novo staining for LAMB2 (G), agrin (H), and COL4A4 (I) increased at day 28 FSGS compared with baseline. The number of mice quantitated is shown below each graph. J−L: graphs of quantification of fluorescence intensity. Fluorescence intensity was increased for LAMB2 (J), agrin (K), and COL4A4 (L) in abnormal glomeruli in FSGS. Original magnification: ×400. Scale bars = 20 μm.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy

    doi: 10.1152/ajprenal.00266.2019

    Figure Lengend Snippet: Staining for the podocyte extracellular matrix proteins laminin-β2 (LAMB2), agrin, and collagen type IV-α2 (COL4A4) are increased in parietal epithelial cells (PECs) in experimental focal segmental glomerulosclerosis (FSGS). A−C: mice at baseline. LAMB2 (A), agrin (B), and COL4A4 (C) immunofluorescent staining (green) was limited to the glomerular basement membrane (yellow arrowheads). Nuclei were stained with DAPI (blue). D−F: experimental FSGS in mice. De novo immunofluorescent staining for LAMB2 (D), agrin (E), and COL4A4 (F) was detected in PECs at day 28 (D28) FSGS (white arrows). G−I: quantification. The percentage of glomerular cross-sections with de novo staining for LAMB2 (G), agrin (H), and COL4A4 (I) increased at day 28 FSGS compared with baseline. The number of mice quantitated is shown below each graph. J−L: graphs of quantification of fluorescence intensity. Fluorescence intensity was increased for LAMB2 (J), agrin (K), and COL4A4 (L) in abnormal glomeruli in FSGS. Original magnification: ×400. Scale bars = 20 μm.

    Article Snippet: The C4 (mouse anti-human LAMB2) antibody was obtained from the Developmental Studies Hybridoma Bank (deposited by J.R. Sanes), developed under the auspices of Eunice Kennedy Shriver National Institute of Child Health and Human Development and maintained by the Department of Biology, The University of Iowa, Iowa City, IA.

    Techniques: Staining, Membrane, Fluorescence

    Double staining confirmed laminin-β2 (LAMB2) expression in parietal epithelial cells (PECs) in mice with experimental focal segmental glomerulosclerosis (FSGS). A and B: double staining for nephrin (red) and LAMB2 (green). A and A3: a normal mouse glomerulus. The merged image shows staining for LAMB2 (green) in the glomerular basement membrane distribution, with nephrin staining (red) alongside. A1 and A2: staining for nephrin (A1) and LAMB2 (A2) showing their absence along Bowman’s capsule. B and B3: FSGS. The merged image shows de novo LAMB2 but not nephrin staining along Bowman’s capsule. B1 and B2: nephrin (B1) and LAMB2 (B2) staining. Higher magnification of the inset in B showed no staining for nephrin (B4) along Bowman’s capsule in areas staining for LAMB2 (white arrows) (B5). Their merged image is shown in B6. C and D: double staining for β-Gal (red) and LAMB2 (green). C and C3: a normal glomerulus. The merged image shows that there was no overlap of LAMB2 staining in the glomerular basement membrane distribution and β-Gal, which permanently labels PECs. C1 and C2: β-Gal and LAMB2. D and D3: FSGS. The merged image shows de novo staining for LAMB2 in a Bowman’s capsule distribution (D1 and D2). Higher power magnification of the inset D shows that β-Gal (yellow arrows) (D4) is lined by LAMB2 (white arrows) (D5), as easily seen in the merged image (D6). Original magnification: ×400. Scale bars = 20 μm.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy

    doi: 10.1152/ajprenal.00266.2019

    Figure Lengend Snippet: Double staining confirmed laminin-β2 (LAMB2) expression in parietal epithelial cells (PECs) in mice with experimental focal segmental glomerulosclerosis (FSGS). A and B: double staining for nephrin (red) and LAMB2 (green). A and A3: a normal mouse glomerulus. The merged image shows staining for LAMB2 (green) in the glomerular basement membrane distribution, with nephrin staining (red) alongside. A1 and A2: staining for nephrin (A1) and LAMB2 (A2) showing their absence along Bowman’s capsule. B and B3: FSGS. The merged image shows de novo LAMB2 but not nephrin staining along Bowman’s capsule. B1 and B2: nephrin (B1) and LAMB2 (B2) staining. Higher magnification of the inset in B showed no staining for nephrin (B4) along Bowman’s capsule in areas staining for LAMB2 (white arrows) (B5). Their merged image is shown in B6. C and D: double staining for β-Gal (red) and LAMB2 (green). C and C3: a normal glomerulus. The merged image shows that there was no overlap of LAMB2 staining in the glomerular basement membrane distribution and β-Gal, which permanently labels PECs. C1 and C2: β-Gal and LAMB2. D and D3: FSGS. The merged image shows de novo staining for LAMB2 in a Bowman’s capsule distribution (D1 and D2). Higher power magnification of the inset D shows that β-Gal (yellow arrows) (D4) is lined by LAMB2 (white arrows) (D5), as easily seen in the merged image (D6). Original magnification: ×400. Scale bars = 20 μm.

    Article Snippet: The C4 (mouse anti-human LAMB2) antibody was obtained from the Developmental Studies Hybridoma Bank (deposited by J.R. Sanes), developed under the auspices of Eunice Kennedy Shriver National Institute of Child Health and Human Development and maintained by the Department of Biology, The University of Iowa, Iowa City, IA.

    Techniques: Double Staining, Expressing, Staining, Membrane

    The podocyte-specific extracellular matrix proteins laminin-β2 (LAMB2), agrin, and collagen type IV-α4 (COL4A4) increased in parietal epithelial cells (PECs) in human focal segmental glomerulosclerosis (FSGS). A–C: LAMB2 (green) and DAPI (blue). A: LAMB2 localized to the glomerular basement membrane (GBM) in the normal human kidney. B and C: two representative glomeruli from different patients with FSGS showing LAMB2 staining along Bowman’s capsule. B′ and C′: higher magnification of the insets showing LAMB2 staining (white arrows) along Bowman’s capsule. D–F: agrin (green) and DAPI. D: agrin localized to the GBM in a normal human glomerulus. E and F: two representative glomeruli from different patients with FSGS showing agrin staining along Bowman’s capsule. E′ and F′: higher magnification of the insets showing LAMB2 staining (white arrows) along Bowman’s capsule. G–I: COL4A4 (green) and DAPI. G: COL4A4 localized to the GBM in a normal human glomerulus. H and I: two representative glomeruli from different patients with FSGS showing COL4A4 staining along Bowman’s capsule. H′ and I′: higher magnification of the insets showing COL4A4 staining (white arrows) along Bowman’s capsule. These results show that podocyte-specific extracellular matrix proteins are expressed de novo by PECs in human FSGS. Original magnification: ×200. Scale bars = 100 μm.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy

    doi: 10.1152/ajprenal.00266.2019

    Figure Lengend Snippet: The podocyte-specific extracellular matrix proteins laminin-β2 (LAMB2), agrin, and collagen type IV-α4 (COL4A4) increased in parietal epithelial cells (PECs) in human focal segmental glomerulosclerosis (FSGS). A–C: LAMB2 (green) and DAPI (blue). A: LAMB2 localized to the glomerular basement membrane (GBM) in the normal human kidney. B and C: two representative glomeruli from different patients with FSGS showing LAMB2 staining along Bowman’s capsule. B′ and C′: higher magnification of the insets showing LAMB2 staining (white arrows) along Bowman’s capsule. D–F: agrin (green) and DAPI. D: agrin localized to the GBM in a normal human glomerulus. E and F: two representative glomeruli from different patients with FSGS showing agrin staining along Bowman’s capsule. E′ and F′: higher magnification of the insets showing LAMB2 staining (white arrows) along Bowman’s capsule. G–I: COL4A4 (green) and DAPI. G: COL4A4 localized to the GBM in a normal human glomerulus. H and I: two representative glomeruli from different patients with FSGS showing COL4A4 staining along Bowman’s capsule. H′ and I′: higher magnification of the insets showing COL4A4 staining (white arrows) along Bowman’s capsule. These results show that podocyte-specific extracellular matrix proteins are expressed de novo by PECs in human FSGS. Original magnification: ×200. Scale bars = 100 μm.

    Article Snippet: The C4 (mouse anti-human LAMB2) antibody was obtained from the Developmental Studies Hybridoma Bank (deposited by J.R. Sanes), developed under the auspices of Eunice Kennedy Shriver National Institute of Child Health and Human Development and maintained by the Department of Biology, The University of Iowa, Iowa City, IA.

    Techniques: Membrane, Staining

    Differential immunofluorescent immunostaining in parietal epithelial cells (PECs) for laminin-β2 (LAMB2), agrin, and collagen type IV-α4 (COL4A4) in an experimental diabetic nephropathy model. A−C: nondiabetic wild-type (WT) mice. Immunofluorescent staining for LAMB2 (A), agrin (B), and COL4A4 (C) staining (green) showed a podocyte distribution. Nuclei were stained with DAPI (blue). D−F: diabetic ob/ob mice. De novo staining was detected in PECs (white arrows) for LAMB2 (D), agrin (E), and COL4A4 (F) and in the mesangium (yellow arrows). G−I: quantification. The percentage of glomerular cross sections with de novo expression along Bowman’s capsule (BC) increased in diabetic mice for LAMB2 (G) and agrin (H) but not COL4A4 (I). Original magnification: ×400. Scale bars = 20 μm.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy

    doi: 10.1152/ajprenal.00266.2019

    Figure Lengend Snippet: Differential immunofluorescent immunostaining in parietal epithelial cells (PECs) for laminin-β2 (LAMB2), agrin, and collagen type IV-α4 (COL4A4) in an experimental diabetic nephropathy model. A−C: nondiabetic wild-type (WT) mice. Immunofluorescent staining for LAMB2 (A), agrin (B), and COL4A4 (C) staining (green) showed a podocyte distribution. Nuclei were stained with DAPI (blue). D−F: diabetic ob/ob mice. De novo staining was detected in PECs (white arrows) for LAMB2 (D), agrin (E), and COL4A4 (F) and in the mesangium (yellow arrows). G−I: quantification. The percentage of glomerular cross sections with de novo expression along Bowman’s capsule (BC) increased in diabetic mice for LAMB2 (G) and agrin (H) but not COL4A4 (I). Original magnification: ×400. Scale bars = 20 μm.

    Article Snippet: The C4 (mouse anti-human LAMB2) antibody was obtained from the Developmental Studies Hybridoma Bank (deposited by J.R. Sanes), developed under the auspices of Eunice Kennedy Shriver National Institute of Child Health and Human Development and maintained by the Department of Biology, The University of Iowa, Iowa City, IA.

    Techniques: Immunostaining, Staining, Expressing

    Laminin-β2 (LAMB2), agrin, and collagen type IV-α4 (COL4A4) staining in human diabetic nephropathy. A–C: LAMB2 staining. A: LAMB2 staining was restricted to the glomerular basement membrane (GBM) in the normal glomerulus. B and C: LAMB2 was detected along Bowman’s capsule in glomeruli from two different patients with diabetic nephropathy. B′ and C′: higher magnification of the insets showing de novo staining for LAMB2 along Bowman’s capsule (white arrows) and in the mesangium (yellow arrow). D–F: agrin staining. D: agrin staining localized to the GBM in the normal glomerulus. E and F: agrin was also detected along Bowman’s capsule in glomeruli from two different patients. E′ and F′: higher magnification of the insets showing staining for agrin along Bowman’s capsule (white arrows) and in the mesangium (yellow arrow). G–I: COL4A4 staining. G: COL4A4 staining localized to the GBM in the normal glomerulus. H and I: COL4A4 was also detected along Bowman’s capsule in glomeruli from two different patients with diabetic nephropathy. H′ and I′: higher magnification showing COL4A4 staining along Bowman’s capsule (white arrows) and in the mesangium (yellow arrow). Original magnification: ×200. Scale bars = 100 μm.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy

    doi: 10.1152/ajprenal.00266.2019

    Figure Lengend Snippet: Laminin-β2 (LAMB2), agrin, and collagen type IV-α4 (COL4A4) staining in human diabetic nephropathy. A–C: LAMB2 staining. A: LAMB2 staining was restricted to the glomerular basement membrane (GBM) in the normal glomerulus. B and C: LAMB2 was detected along Bowman’s capsule in glomeruli from two different patients with diabetic nephropathy. B′ and C′: higher magnification of the insets showing de novo staining for LAMB2 along Bowman’s capsule (white arrows) and in the mesangium (yellow arrow). D–F: agrin staining. D: agrin staining localized to the GBM in the normal glomerulus. E and F: agrin was also detected along Bowman’s capsule in glomeruli from two different patients. E′ and F′: higher magnification of the insets showing staining for agrin along Bowman’s capsule (white arrows) and in the mesangium (yellow arrow). G–I: COL4A4 staining. G: COL4A4 staining localized to the GBM in the normal glomerulus. H and I: COL4A4 was also detected along Bowman’s capsule in glomeruli from two different patients with diabetic nephropathy. H′ and I′: higher magnification showing COL4A4 staining along Bowman’s capsule (white arrows) and in the mesangium (yellow arrow). Original magnification: ×200. Scale bars = 100 μm.

    Article Snippet: The C4 (mouse anti-human LAMB2) antibody was obtained from the Developmental Studies Hybridoma Bank (deposited by J.R. Sanes), developed under the auspices of Eunice Kennedy Shriver National Institute of Child Health and Human Development and maintained by the Department of Biology, The University of Iowa, Iowa City, IA.

    Techniques: Staining, Membrane

    Podocyte-specific proteins colocalize with the activated parietal epithelial cell (PEC) marker CD44 in mice with experimental focal segmental glomerulosclerosis (FSGS). Double staining was performed for CD44 (red) and podocyte-specific extracellular matrix proteins (green). A−C: normal mice. The merged images show staining for laminin-β2 (LAMB2; A), agrin (B), and collagen type IV-α4 (COL4A4; C) but not CD44 in normal glomeruli. A1−C3: individual stains for LAMB2 (A1−A3), agrin (B1−B3), and COL4A4 (C1–C3). D−F: FSGS mice. The merged images show colocalization (yellow) of CD44 in PECs with LAMB2 (D and D3) and agrin (E and E3) but not COL4A4 (F and F3). D1, D2, E1, E2, F1, and F2: corresponding single stainings for LAMB2 (D1 and D2), agrin (E1 and E2), and COL4A4 (F1 and F2). Single and merged higher magnifications of the insets are shown in D4–F6). These results are consistent with activated PECs expressing the podocyte-specific extracellular matrix proteins LAMB2 and agrin in FSGS. Original magnification: ×400. Scale bars = 20 μm.

    Journal: American Journal of Physiology - Renal Physiology

    Article Title: Differential expression of parietal epithelial cell and podocyte extracellular matrix proteins in focal segmental glomerulosclerosis and diabetic nephropathy

    doi: 10.1152/ajprenal.00266.2019

    Figure Lengend Snippet: Podocyte-specific proteins colocalize with the activated parietal epithelial cell (PEC) marker CD44 in mice with experimental focal segmental glomerulosclerosis (FSGS). Double staining was performed for CD44 (red) and podocyte-specific extracellular matrix proteins (green). A−C: normal mice. The merged images show staining for laminin-β2 (LAMB2; A), agrin (B), and collagen type IV-α4 (COL4A4; C) but not CD44 in normal glomeruli. A1−C3: individual stains for LAMB2 (A1−A3), agrin (B1−B3), and COL4A4 (C1–C3). D−F: FSGS mice. The merged images show colocalization (yellow) of CD44 in PECs with LAMB2 (D and D3) and agrin (E and E3) but not COL4A4 (F and F3). D1, D2, E1, E2, F1, and F2: corresponding single stainings for LAMB2 (D1 and D2), agrin (E1 and E2), and COL4A4 (F1 and F2). Single and merged higher magnifications of the insets are shown in D4–F6). These results are consistent with activated PECs expressing the podocyte-specific extracellular matrix proteins LAMB2 and agrin in FSGS. Original magnification: ×400. Scale bars = 20 μm.

    Article Snippet: The C4 (mouse anti-human LAMB2) antibody was obtained from the Developmental Studies Hybridoma Bank (deposited by J.R. Sanes), developed under the auspices of Eunice Kennedy Shriver National Institute of Child Health and Human Development and maintained by the Department of Biology, The University of Iowa, Iowa City, IA.

    Techniques: Marker, Double Staining, Staining, Expressing